MULTIPLEX SYBR® GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS

MULTIPLEX SYBR® GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS

Blog Article

A novel SYBR® green-real time polymerase chain reaction (qPCR) Screens was developed to detect two Bartonella species, B.henselae and B.clarridgeiae, directly from blood samples.The test was used in blood samples obtained from cats living in animal shelters in Southern Brazil.Results were compared with those obtained by conventional PCR targeting Bartonella spp.

Among the 47 samples analyzed, eight were positive using the conventional PCR and 12 were positive using qPCR.Importantly, the new qPCR detected the presence of both B.henselae and B.clarridgeiae in two samples.The results show that the qPCR described here may be a reliable Motorcycle Kit tool for the screening and differentiation of two important Bartonella species.